High content live cell assays from 96 wells to single cells

 

A label-free high-content system

Livecyte changes what's possible to measure using live cell assays

 

  • Livecyte produces exceptionally high contrast time-lapse videos using Phasefocus’s patented Ptychographic quantitive phase imaging (QPI) technology for a range of label-free assays with or without up to seven channels of complementary fluorescence.
  • Automated single-cell tracking of even the most sensitive cells quickly reveals subtle phenotypic differences in unperturbed cell populations.
  • Easy-to-use dashboards present coherent and concise results from up to 96 wells at a time whilst retaining the ability to investigate individual cell behaviour and outlying characteristics.

 

 

From Images to Insights

 

  • Easy-to-use image processing and tracking algorithms allow individual cells to be identified in real time.
  • Explore how the properties of those identified cells, and how they change over time.

 

 
 

 

Powerful Integrated Analysis

 

  • Integrated analysis suite enables cell behaviour to be monitored as a function of treatment
  • Easily export results in Excel and Graphpad formats
  • Monitor many different types of behaviour, from morphological to motile, in every experiment
  • No need for multiple separate experiments

 

 

Find out more about Livecyte applications

From the assay level...

...To the single-cell level

 

Assay-Level Analysis

 

  • Large fields of view (up to 4mm2) in an assay relevant plate format (up to 96 well plate) mean the behaviour of many cells can be captured making results statistically robust.
  • Unlike other QPI systems, Livecyte's ptychography technology removes the need for plate inserts or expensive consumables - reducing running costs compared to comparative systems.
  • Correlate changes in proliferation, motility and morphology as a function of treatment, from every experiment.

 

Screenshot of example Livecyte outputs

 

 

 

Explore Population Heterogeneity

 

  • Identify, track and classify individuals and monitor their phenotypic behaviour over time.
  • Classify subpopulations based of any combination of parameters.
  • Compare subpopulation behaviour as a function of treatment.
Plot Showing Heterogeneity of Cell Velocity
Example Fluorescence-gated Image

 

 

 

Single-cell Lineage

  • Automatically segment and track individuals and identify their division events and children.
  • Quick and easy track correction enables perfect lineage determination.
  • Explore single cell or whole lineage phenotypical properties across multiple generations.
Example Lineage Tracing Screenshot