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Livecyte®

Kinetic Cytometer

High content live cell assays from 96 wells to single cells

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A label-free high-content system

Livecyte changes what's possible to measure using live cell assays

 

  • Livecyte produces exceptionally high contrast time-lapse videos using Phasefocus’s patented Ptychographic quantitive phase imaging (QPI) technology for a range of label-free assays with or without up to seven channels of complementary fluorescence.
  • Automated single-cell tracking of even the most sensitive cells quickly reveals subtle phenotypic differences in unperturbed cell populations.
  • Easy-to-use Dashboards present coherent and concise results from up to 96 wells at a time whilst retaining the ability to investigate individual cell behaviour and outlying characteristics.

 

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From Images to Insights

 

  • Easy-to-use image processing and tracking algorithms allow individual cells to be identified in real time.
  • Explore how the properties of those identified cells, and how they change over time.

 

 
 

 

Powerful Integrated Analysis

 

  • Integrated analysis suite enables cell behaviour to be monitored as a function of treatment
  • Easily export results in Excel and Graphpad formats
  • Monitor many different types of behaviour, from morphological to motile, in every experiment making laboratory time more productive 

 

 

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From the assay level...

...To the single-cell level

 

Assay-Level Analysis

 

  • Large fields of view (up to 4mm2) in an assay relevant plate format (up to 96 well plate) mean the behaviour of many cells can be captured making results statistically robust.
  • Unlike other QPI systems, Livecyte's ptychography technology removes the need for plate inserts or expensive consumables - reducing running costs compared to comparative systems.
  • Correlate changes in proliferation, motility and morphology as a function of treatment, from every experiment.

 

Screenshot of example Livecyte outputs

Example outputs from a motility dose response assay

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Explore Population Heterogeneity

 

  • Identify, track and classify individuals and monitor their phenotypic behaviour over time.
  • Classify subpopulations based of any combination of parameters.
  • Compare subpopulation behaviour as a function of treatment.

 

Mitotic cells identified from their high thickness/low area phenotype

Example Fluorescence-gated Image

Subpopulation of cells highlighted based on mCherry expression.

 

Single-cell Lineage

  • Automatically segment and track individuals and identify their division events and children.
  • Quick and easy track correction enables perfect lineage determination.
  • Explore single cell or whole lineage phenotypical properties across multiple generations.
Example Lineage Tracing Screenshot

A comparison of the growth rates of two lineages within the same population

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Technical Specifications

Imaging Modalities Brightfield; Ptychographic quantitative phase imaging; Fluorescence (up to 7 channels)
Compatible Plate Formats Standard 6, 12, 24, 48, 96 well plates (glass and high-grade plastic), 35mm dishes, slides (inc. Ibidi µ-slides)
Required Plate Inserts None
Objective Lens Options 4x; two from 10x, 20x or 40x (Plan N, Fluorite and UPLXAPO options available, air only)
Camera High sensitivity sCMOS (2048 x 2048 pixels; 6.5µm pixel size; 16 bit; 82% Peak QE) 
XY Stage Automatic Linear-encoded ASI stage 
Z Stage Automatic Olympus Z-positioner
Ptychographic QPI Illumination Integrated low-power laser diode (typically 30µW/mm2 at 650nm wavelength)
Fluorescence Illumination CoolLED pE-300 or CoolLED pE-4000 options available
Fluorescence Filters System compatible with up to 7 channels (in addition to QPI modality)
QPI Field of View Up to 2 x 2mm with 10x lens
Lateral Resolution Up to 0.4µm with a UPLXAPO 20x
QPI Capture Rate Up to one image per 13s
Environment Control Integrated stand-alone incubator controlling temperature, CO2, humidity and evaporation
IT Bespoke Acquisition PC, Analysis PC and Network Attached Storage (up to 40Tb) options
Software Acquire acquisition software, Cell Analysis Toolbox analysis software available
Space Requirements 1800 x 800 x 900 mm (W x D x H)
Weight ~65kg depending on configuration
Power Requirements Nominal 700 W
Gas Requirements Supply of 5% CO2 (in air) either from a cylinder with regulator or from mains supply via a CO2 mixer