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Automated Cell Motility Assays on Livecyte

 

Livecyte removes the need to manually track live cells by combining high contrast label-free imaging with automatic cell tracking algorithms to directly measure cell motile behaviour at the single cell level. This enables users to…

  • Overcome the problems with manually tracking live cells
  • Automatically generate motility outputs without introducing phototoxcity
  • Directly measure cell motility without the need for proxy gap closure assays

 

 

Manually tracking live cells is prone to error

 

It is a common misconception that because a cell has been tracked manually by a human that resulting motility information is then accurate.

 

Single pixel errors in mouse click position during manual tracking can add up to significant errors in track length and cell velocity.

 

A lack of standardisation in technique creates substantial variability between users and poor repeatability making results less reproducible.

Read the inconvenient truth about manual tracking

 

 

Automatically generate motility outputs, without phototoxcity

 

  • Livecyte’s quantitative phase imaging (QPI) generates high-contrast images without the need for fluorescent labels.
  • These images are ideal for automatic segmentation and tracking.
  • This enables users to repeatably and accurately measure cell motility automatically without having to introduce toxic fluorescent labelling.
 

 

 

Directly measure cell motility without gap closure assays

 

Conventional wound closure assays suffer from repeatability issues, ambiguities around the impact of proliferation, cell density effects and provide no indication of heterogeneity

 

Livecyte directly measures the motility of each individual cell yielding a variety of cell motion parameters such as velocity, displacement, directionality and confinement ratio.

 The Livecyte is excellent for assessing cell behaviour analysis and quantification.  

 

Dr Viviane Mignone, University of Nottingham