Label-free imaging uses inherent contrast mechanisms within cells to create image contrast without
introducing dyes/labels, which may confound results. Quantitative phase imaging is label-free and offers
higher content and contrast compared to traditional techniques. High-contrast images facilitate generation
of individual cell metrics via more robust segmentation and tracking, enabling formation of a
label-free dynamic phenotype describing cell-to-cell heterogeneity and temporal changes. Compared to
population-level averages, individual cell-level dynamic phenotypes have greater power to differentiate
between cellular responses to treatments, which has clinical relevance e.g. in the treatment of cancer.
Furthermore, as the data is obtained label-free, the same cells can be used for further assays or expansion,
of potential benefit for the fields of regenerative and personalised medicine.

 

Click to download PDF : Characterising live cell behaviour Traditional label-free and quantitative phase imaging approaches

 

© 2017 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license
(http://creativecommons.org/licenses/by/4.0/).