Lensless Microscopy Technology

How lensless microscopy works

The lensless microscope illuminates an area of a specimen and records the diffraction pattern.

The illumination is then translated by a fixed step (software controlled) and the process is reapidly repeated (typically 25 – 100 times) to cover the area of interest.

The total area covered is user definable.

Software

Brightfield and phase images are computer from the set of diffraction patterns by the computer lens.

Range of Applications

In principle this method is applicable to the entire electromagnetic spectrum. Other potential applications therefore exist in radar, ultra-violet and infra-red imaging, and terahertz imaging.

Augmented functionality

The technique enables aberration free imaging. It is cost effective because it eliminates the cost of high quality optics (multi-element objectives, EM-lenses and zone plates)

In optical microscopy:

• large working distance
• non-invasive imaging (sterile imaging, incubation imaging)
• high resolution
• quantitative phase information and brightfield imaging
• aberration free imaging (no complicated or compound optics, microscope objectives)
• post-acquisition data processing, optical sectioning and 3D imaging
• user definable field of view
• Structures hidden by light absorption revealed