Measurement of mitotic time is important to numerous fields of cell biology and has uses in the development of anti-mitotic cancer drugs. Typically, manual methods are employed to identify mitotic events, track through cell division and record mitotic time. Such methods are subject to user bias and are time-intensive to obtain a minimal number of data points to ensure statistical significant comparison between different drugs/treatments. The Livecyte system overcomes these issues by combining Quantitative Phase Imaging (QPI) with downstream automated analysis software.
Differences in dynamic and morphological phenotypic characteristics enabled by label free individual cell analysis
A case study to demonstrate how analysis of a population to an individual cell level can reveal subtle differences in cell behaviour that would be unresolved by a population averaged approach.
Characterisation of sub-populations of heterogeneous patient-derived primary prostate epithelial cell cultures
Analyse and extract morphological and dynamic phenotypes to identify heterogeneity within mixed cancer cell populations.
Produce a Staurosporine dose response curve without use of fluorescent labels.
Monitor continuously the mitotic index of a cell population in a non-invasive manner.
Delve deeper into the dynamics of cell proliferation through non-invasive time-lapse imaging with the Livecyte system.
Use non-invasive time-lapse imaging to quantify cell death without labels, dyes or phytotoxic damage.
Go beyond traditional wound healing assays with the Phasefocus Livecyte.